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KMID : 0368019940170041383
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Journal of Soonchunhyang University
1994 Volume.17 No. 4 p.1383 ~ p.1394
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New Selective Medium for Identification of Vibrio vulnificus
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Kim Chang-Se
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Abstract
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For rapid and accurate diagnosis of Vibrio vulnificus infection, selection of better culture media has been contineously pursued. Several kinds of media were developed for favorable growth conditions of V. vulnificus as well as more efficient
differentiation of V. vulnificus from V. parahaemolyticus.
These media which introduced in this report including TCBS-Cellobiose agar plate medium BHI-Cellobiose agar plate medium, Yeast extract-Beef extract-Cellobiose agar plate medium and Meretrix lusoria-Cellobiose agar plate medium were compared in
order to
select the most efficient method for cultivation and differential identification of V.vulnificus and V.parahaemolyticus
We cultivated the V. vulnificus and V.parahaemolyticus on the different kinds of media newly developed and the results were follows.
1. Yeast extract-Beef extract-Cellobiose agar medium is found to be the best for enrichment medium of V.vulnificus and also Meretrix lusoria-Cellobiose agar medium is seem to be comparable.
2. Yeast extract-Beef extract-Cellobiose with 0.8% oxgall and Meretrix lusoria-Cellobiose agar medium is appeared to suitable for differential medium of V.vulnificus.
3. TCBS-Cellobiose agar medium showed potential use of differential medium of V.vulnificus but the growth of V.vulnificus is slightly suppressed on this medium.
4. TCBS agar medium is not suitable for differential medium of V vulnificus .
5. BHI-Cellobiose agar medium was not suitable for differential medium of V.vulnificus because of its suppression of the growth of V.vulnificus.
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